We are happy to announce the publication of our work on modular hemicellulose production in Biotechnology for Biofuels, a leading (open-access!) journal for advancing the biological production of fuels, chemicals, and biomaterials. Here, we describe how enzymes from the cellulose synthase-like superfamily (found throughout the plant kingdom) can be assembled as modular parts (akin to LEGO bricks) to modulate their function and effects on eukaryotic cell growth.
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Despite rising interest in the engineering of polysaccharide-based biomaterials, relatively little is known about how plant hemicelluloses can be built and tailored in non-plant cell factories. Pichia pastoris is a promising host to study cellulose synthase-like A (CSLA; heteromannan synthases) and CSLC (xyloglucan synthase) activities but the functions of specific protein motifs and the impactContinue reading “New preprint on yeast bioengineering to produce modular plant hemicellulose”
We are happy to have contributed to a new publication that appeared in Science Advances on the February 10. This study unveils how jasmonate signaling is initiated in the korrigan1 (kor1) cellulose-deficient mutant, and multiple ways in which it can be complemented. The work was lead by Stefan Mielke in Debora Gasperini’s research group at IPB Halle.
Catalin Voiniciuc performed the cell wall analyses, and would like to acknowledge the valuable technical assistance of Christine Wagner and Bo Yang. The Science Advances study was originally available as a pre-print:
February also marks the release of a correction to the Voiniciuc et al. (2018) Plant Physiology study of pectin biosynthetic enzymes. Unfortunately, there was an accidental mix-up of GAUT11 and another GAUT protein expressed at the same time. Catalin would like to thank the co-authors for reporting the problem affecting Figure 5 and Supplemental Figure 4A, and repeating the experiments. The corrigendum has addressed this issue and shows that the original results and conclusions that GAUT11 is a galacturonosyltransferase (GalAT) that elongates homogalacturonan (HG) are unaffected.
Our revised manuscript on the roles of branched xylan and heteromannan (previously available as a preprint) in controlling seed mucilage properties and early growth in salt stress has now been peer-reviewed.
A collaborative study of mannan polysaccharide production in wheat (Triticum aestivum) is now published in the journal Plant Science. Wheat-like polymers that normally accumulate in the developing endosperm of the grain were produced in two heterologous hosts by the expression of a single enzyme, TaCSLA12. Additional mannan-related genes were identified in the wheat endosperm but were not essential for making the polysaccharides in yeast. Moreover, a wheat-like mannan production in a glucomannan-deficient Arabidopsis thaliana triple mutant (csla2 csla3 csla9) did not alter the plant morphology.
Congratulations to our colleagues at INRAE (Nantes, France; who led the study), the Joint BioEnergy Institute (Berkeley, California), Rothamsted Research (UK), and Heinrich Heine University (Düsseldorf, Germany; where our experimental work was conducted).