The Designer Glycans lab is represented by four members (Catalin, Annika, Bo, and Madalen) at the 7th International Conference on Plant Cell Wall Biology (PCWB2021). Originally scheduled to take place in Sapporo, Japan last year, this meeting is now in a digital format to accommodate a global audience from June 27 to July 1, 2021.
We are happy to see many international colleagues from the plant cell wall biology field and present our latest unpublished results in two posters (Bo and Annika) and one talk (Madalen) during this exciting meeting.
We are happy to announce the publication of our work on modular hemicellulose production in Biotechnology for Biofuels, a leading (open-access!) journal for advancing the biological production of fuels, chemicals, and biomaterials. Here, we describe how enzymes from the cellulose synthase-like superfamily (found throughout the plant kingdom) can be assembled as modular parts (akin to LEGO bricks) to modulate their function and effects on eukaryotic cell growth.
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Two exciting career opportunities are available in the Designer Glycans group starting February 2022. The PhD and postdoctoral positions are fully funded and offer outstanding benefits. Our ambitious independent junior research group has state-of-the-art facilities for carbohydrate analysis, cell imaging and plant cultivation. We are looking for candidates who are interested in synthetic biology, plant cell walls and/or engineered living materials. Both positions will build upon our recent findings, such as:
Despite rising interest in the engineering of polysaccharide-based biomaterials, relatively little is known about how plant hemicelluloses can be built and tailored in non-plant cell factories. Pichia pastoris is a promising host to study cellulose synthase-like A (CSLA; heteromannan synthases) and CSLC (xyloglucan synthase) activities but the functions of specific protein motifs and the impactContinue reading “New preprint on yeast bioengineering to produce modular plant hemicellulose”
Despite rising interest in the engineering of polysaccharide-based biomaterials, relatively little is known about how plant hemicelluloses can be built and tailored in non-plant cell factories. Pichiapastoris is a promising host to study cellulose synthase-like A (CSLA; heteromannan synthases) and CSLC (xyloglucan synthase) activities but the functions of specific protein motifs and the impact of their products remain unclear. Here, we used a Golden Gate-based system to assemble chimeric CSL enzymes and optimize yeast cultivation to rapidly produce hemicellulose in mg scale.
Interestingly, prolonged expression of a konjac AkCSLA3 glucomannan synthase was toxic to yeast cells, but this impairment was restored by swapping its C-terminal region with that of an Arabidopsis AtCSLA2 mannan synthase. Additional chimeras exceeded the yields of the native enzymes. Overall, cell size was increased or decreased depending on the CSLA sequences used.
Yeast containing plant-like polysaccharides could be viewed as tunable biological capsules for further metabolic engineering studies. Sensitive macromolecules such as therapeutic proteins can be protected by encapsulation in non-toxic plant polysaccharides and Pichia cells are attractive hosts for recombinant protein production. Therefore, our strategy provides basic insight into matrix polysaccharide biosynthesis and could also be used to encapsulate valuable cargo.