Designer Glycans

Making and Breaking Cell Walls with Precision

Wheat is one of the most important cereal crops on the planet. Here, we investigated the wheat enzymes are responsible for synthesizing mannan, a dietary fiber.

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New publication on wheat mannan

A collaborative study of mannan polysaccharide production in wheat (Triticum aestivum) is now published in the journal Plant Science. Wheat-like polymers that normally accumulate in the developing endosperm of the grain were produced in two heterologous hosts by the expression of a single enzyme, TaCSLA12. Additional mannan-related genes were identified in the wheat endosperm but were not essential for making the polysaccharides in yeast. Moreover, a wheat-like mannan production in a glucomannan-deficient Arabidopsis thaliana triple mutant (csla2 csla3 csla9) did not alter the plant morphology.

Congratulations to our colleagues at INRAE (Nantes, France; who led the study), the Joint BioEnergy Institute (Berkeley, California), Rothamsted Research (UK), and Heinrich Heine University (Düsseldorf, Germany; where our experimental work was conducted).

Read more at https://doi.org/10.1016/j.plantsci.2020.110693

Fig. 2 from the study. Subcellular localization of enzymes that may be involved in mannan production. (Source: https://doi.org/10.1016/j.plantsci.2020.110693)

Fig. 3 from the study. Quantification of beta-mannan production in yeast cells after plant enzyme expression. (Source: https://doi.org/10.1016/j.plantsci.2020.110693)

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