We published a review article in Plants (https://doi.org/10.3390/plants8110516), part of a special issue on “Plant Cell Wall Biology”. In close collaboration with multiple colleagues from Düsseldorf, we assessed the suitability of more than ten orthogonal hosts for synthetic biology and to study plant cell wall biosynthesis.
In particular, we focused on the key advantages and limitations of several bacterial, fungal and animal systems for the heterologous expression of plant glycosyltransferases to reconstruct polymers. We also analyzed the glycosidic linkages of native polymers from promising orthogonal hosts and discuss how they might impact the heterologous production of various plant polysaccharides.
Pauly M, Gawenda N, Wagner C, Fischbach P, Ramírez V, Axmann IM, Voiniciuc C* (2019) The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis. Plants 8: 516